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The staining results were visualized using an Axio Observer7 microscope (Carl Zeiss Meditec AG, Jena, Germany)
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Gluten and casein-derived molecules, if present in subjects with altered immune system, like in ASD, can cross the highly permeable intestinal barrier, and activate pro-inflammatory processes, releasing pro-inflammatory mediators that travel to the brain centers through the blood, contributing to neuro-inflammatory activities ( 5 Antioxidants targeting oxidative stress and neuroinflammation in ASD Besides excessive levels of oxidative stress and pro-inflammatory molecules, ASD patients are also distinguished for their nutritional deficits